ABSTRACT
A patient with paraquat poisoning was followed up for five years, and it was showed that the interstitial lesion areas in chest CT of this patient gradually decreased after acute period of the poisoning and no significant changes were found six months later. After that the density of the lesions gradually reduced, while the cystic air cavities slowly increased. In addition, the patient's exercise endurance gradually improved over time, and the lung function was close to the normal level five years after poisoning. The follow-up report helps clinicians to have a deeper understanding of the long-term outcome of paraquat poisoning.
Subject(s)
Humans , Follow-Up Studies , Lung/pathology , Paraquat , Poisoning , Tomography, X-Ray ComputedABSTRACT
OBJECTIVE@#To investigate the protective effects and underlying mechanisms of Xuebijing Injection (XBJ) on the lung endothelial barrier in hydrogen sulfide (H@*METHODS@#Sprague-Dawley rats were exposed to H@*RESULTS@#The morphological investigation showed that XBJ attenuated H@*CONCLUSIONS@#XBJ ameliorated H
Subject(s)
Animals , Rats , Claudin-5 , Drugs, Chinese Herbal , Endothelial Cells , Hydrogen Sulfide , Phosphatidylinositol 3-Kinases , Rats, Sprague-Dawley , Respiratory Distress Syndrome, Newborn/drug therapyABSTRACT
<p><b>OBJECTIVE</b>To investigate the early prognostic values of arterial lactate and base excess (BE) in patients with paraquat poisoning.</p><p><b>METHODS</b>Seventy-five patients with paraquat poisoning were divided into sudden death group (n = 10) who died within 24 h after admission, recent death group (n = 31) who died more than 24 h after admission, and survival group (n = 34). Arterial lactate and BE were measured on admission and at 24 h after admission. The prognostic values of arterial lactate and BE were analyzed.</p><p><b>RESULTS</b>The arterial lactate measured on admission was significantly higher in the sudden death group than in the recent death group and survival group (P < 0.01), but there was no significant difference in arterial lactate between the recent death group and survival group (P = 0.309). The BE measured on admission was significantly lower in the sudden death group than in the recent death group and survival group, and it was significantly lower in the recent death group than in the survival group (P < 0.01 or P < 0.05). At 24 h after admission, the recent death group had a significantly higher arterial lactate (P < 0.01) and a significantly lower BE (P < 0.01), as compared with the survival group. The logistic regression analysis showed that the two indices were significantly associated with prognosis (P < 0.01). On admission, the areas under the receiver operating characteristic (ROC) curve (AUCs) of arterial lactate and BE for predicting death were 0.692 and 0.787, respectively, and the cut-off values were 3.25 mmol/L and -1.75 mmol/L, respectively; the AUCs of arterial lactate and BE for predicting sudden death were 0.995 and 1, respectively, and the cut-off values were 7.1 mmol/L and -12.8 mmol/L, respectively. At 24 h after admission, the AUCs of arterial lactate and BE for predicting death were 0.743 and 0.822, respectively, and the cut-off values were 2.15 mmol/L and -5.55 mmol/L, respectively.</p><p><b>CONCLUSION</b>Arterial lactate and BE have certain values in predicting the death, especially the sudden death, in patients with acute paraquat poisoning.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Arteries , Chemistry , Lactic Acid , Blood , Paraquat , Poisoning , Poisoning , Diagnosis , PrognosisABSTRACT
<p><b>OBJECTIVE</b>To observe vasodilation effect of EUL and its mechanism.</p><p><b>METHOD</b>Isometric tension was recorded in isolated rat thoracic artery precontracted by noradrenaline (NE) to study the vasodilation effect of EUL, and to investigate the role of endothelial cell and vascular smooth muscle cell on vasodilation.</p><p><b>RESULT</b>EUL was shown to significantly relax the endothelium-intact arteries precontracted by NE, relaxation had fall down on endothelium-denuded arteries. EUL did not affect the concentration-contraction curve of K+. NE and CaCl2 to shift to the right with an decrease in the maximum effective response, manifesting non-competitive antagonism (P <0. 01). The relaxant effect of EUL was significantly inhibited by pretreatment of endothelium-denuded aorta with potassium channel antagonist glibenclamide.</p><p><b>CONCLUSION</b>EUL induces relaxation in rat aortic rings. The mechanisms may involve the endothelium and the activation of the potassium channels.</p>
Subject(s)
Animals , Female , Male , Rats , Aorta, Thoracic , Physiology , Dose-Response Relationship, Drug , Endothelium, Vascular , Physiology , Eucommiaceae , Chemistry , Glyburide , Pharmacology , Lignans , Pharmacology , Muscle Contraction , Norepinephrine , Pharmacology , Plants, Medicinal , Chemistry , Potassium Channel Blockers , Pharmacology , Rats, Sprague-Dawley , Vasodilation , Vasodilator Agents , PharmacologyABSTRACT
The production conditions of glutathione with shaking flask fermentation by the mutant Saccharomyces J-X25, a methionine-defected strain were studied, and the optimum culture conditions are as follows: initial pH6. 0, temperature 30℃, 100ml in 500ml flask, the inoculum size 10% and agitation rate 220r/min. The emphasis was on the stimulating effect on the cells by dioxogen and the sodium lactate as surfactant. Both of which were added at the logarithmic phase of fermentation, and the GSH production was up to 0. 253g/L , 52% higher than the control without the additions. Compared with the production of GSH initial strain that by the mutant in optimum conditions was increased by 79%.